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LIBRARY OF CONGRESS. 

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ANIMAL VACCINATION. 



VACCINATION 






freventive ivemech 






AGAINST 



DISEASES OF LIVE 



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;:7V7_:^H7zz s- 



HAROLD SORBY 



3-1895- f 

3^// ! 



9 



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A 



INTRODUCTION. 



V\ 



Anthrax appears to exist in the United States and Canada to a greater 
extent than is generally supposed. The dangerous and fatal character of 
this contagious disease is only too well known. 

It will be conceded that if the owners of live stock can effectually pro- 
tect their flocks and herds against one of the most deadly diseases to which 
they are always exposed, much will be accomplished. Thanks to Louis 
Pasteur, protec&on against anthrax can be obtained by the simple process 
of "vaccination." 

Pasteur discovered a "vaccine" against anthrax in 1882. Beginning with 
France the European countries have, one after the other, adopted Pasteur's 
system of vaccination, and now the Anthrax Vaccine seems destined to 
become universally used in Europe. Australia is following and deriving 
benefit from its commefndable course. 

Pasteur's Anthrax Vaccine has now found its way across the North 
Atlantic and we thine that the American and Canadian farmer and stock- 
owner will be quick t> realize the advantages of vaccination. The leading 
veterinarians have alnady expressed their approval of preventive inoculation 
for anthrax in domesic animals, and the introduction of Pasteur's system 
will, we hope, meet vth the hearty co-operation of veterinary surgeons in 
all parts of the Nortl American Continent. 

HAROLD SORBY. 

New York, May, 185 



\ 






I.— LOUIS PASTEUR. 



HIS WONDERFUL DISCOVERIES. 

Louis Pasteur, the French scientist, whose name and discoveries are 
known all over the world, was born in 1822, and at an early age began to 
devote himself to physical science. His career has been one of uninter- 
rupted success, and during the last 40 years honors have been showered 
upon him. M. Pasteur's principal discoveries have been in connection 
with microscopic organisms. He studied the phenomena of fermentation 
and proved that ferments were living things. He rendered incalculable 
service by revealing the cause and devising means of preventing the dis- 
orders of wine and beer and milk. He proved the fallacy of spontaneous 
generation and demonstrated the germ theory of disease. Pasteur saved 
the silk industry from ruin; he discovered the cause df disease in silkworms 
and the mode of dealing with them. 

In 1S76 Pasteur began his investigation of the rkcillus anthracis — the 
germ of the disease known as anthrax, splenic fever, : lalignant pustule, etc. 
(Ger. : Milzbrand; Fr.: Charbon), and which comr its its ravages in all 
parts of the world. His announcement of the diiiovery of a vaccine 
against this mortal disease was enthusiastically rec ived in 1 



Pasteur's more recent discoveries and researches 



hydrophobia are fresh in the mind of every one; ^id the latest achieve- 

diphtheric Serum — 

T|ie Pasteur Institute 

worthy memorial tc 

he Institute we may 



ment of the Paris Pasteur Institute — Dr. Roux's An 

is already too well known to need comment. 

founded in Paris by public subscription in 1888, is 

the great scientist; and from within the precincts of 

expect further important discoveries from the emii-nt men who, led by 

Messrs. Chamberland and Roux, are, like Pasteur, c voting their lives to 

scientific research. 



n regard to rabies or 



4 
II.— ANTHRAX. 



Anthrax is a dangerous, contagious and fatal disease. It exists in all 
parts of the world. It affects most animals, especially herbivora, particu- 
larly cattle, sheep, horses, mules, goats and swine. The disease is com- 
municable to man, being known as malignant pustule and woolsorters* 
disease. It manifests itself in different forms, varying with the country 
and the species of animal. Anthrax is or has been known under a 
variety of names, particularly: splenic apoplexy, gangrene of the spleen, 
anthrax fever, splenic fever, apoplectic anthrax, malignant anthrax, malig- 
nant carbuncle, carbuncular fever, bloody murrain, black murrain, black 
quarter, *black leg, bloodstriking, braxy, etc. It is also known as Charbon 
l'rVan< ej\ .w'.'l/brand (Germany), Cumberland disease (Australia), Siberian 
pesl ( Russia). Lcodiana disease (India), etc. 

Some of the above names are no longer used to express true anthrax, 
yet there is little doubt but that deaths from anthrax have been erroneously 
attributed to some of the above-named diseases. 

Anthrax is caused by the introduction of a germ into the body, usually 
with the food. The sources of contagion are innumerable. The pasture 
may be infected by the proximity of graves of animals that have died of 
anthrax or by the ante-mortem or post-mortem discharges of the diseased 
animal. The germ remains alive in the ground for several years, and cattle 
grazing over anthrax graves will invariably contract the disease; a stream 
passing near such graves will scatter the germs everywhere along its course; 
fodder from an infected field will occasion an outbreak; manure is a 
constant source of infection. The malady is also caused by actual contact, 
the germ entering the body through scratches, sores or wounds. Moreover 
it is believed that Hies carry the germ. 

The innumerable channels by which anthrax may be introduced upon 
a farm or range causes the danger of an outbreak to be always present. In 
many cases the soiine is quite unaccountable, and the disease appears, 
apparently, spontanec usly. Again, the difficulty of effectually disposing of 
carcasses and of thi roughly disinfecting the surroundings renders the 
recurrence of an outlreak of anthrax almost inevitable. All sudden deaths 
or heavy mortality ii cattle, sheep, horses, mules and goats should be 
suspected as being caised by anthrax. 

Owing to the rajidly fatal termination of a case of anthrax — death 
following a few hoirs' sickness — treatment is unsatisfactory and even 
impossible, especially at the beginning of an outbreak. Hence the im- 
portance of a. prevents remedy. 

In studying anthrx we must remember that the disease is communicable 

to man, and every ye.- we hear of a large number of deaths from malignant 

pustule and woolsorrs' disease among veterinarians, butchers, tanners, 

■ r-. and othersengaged in tending or handling domestic animals 

* See n< \-e on page 13 regard to Special Black Leg Vaccine. 




The germ of anthrax is known as the bacillus anthracis. It is a short, 
straight filament or rod, detached and motionless, visible only through a 

microscope and magnified to 400 
or 500 diameters. 

Prior to Pasteur s researches it 
was extremely difficult to diagnose 
anthrax. Characteristic symptoms 
were often at fault and the prac- 
titioner undecided. Thanks to 
Pasteur s discoveries there is no 
longer any possibility of doubt: 
whenever anthrax bacilli are found 
in the blood of an animal recently 
dead, anthrax was the cause of 
death; whenever, on the contrary, 
no such bacilli are present, the 
animal succumbed to another 
disease. The presence of these ba- 
cilli is the only proof of anthrax. 
Fig. 1 represents the blood of a healthy animal, magnified to 400 
diameters. 

Fig. 2 represents the blood of an animal which has died of anthrax. 
The globules of blood have lost their roundness; but this character of the 
globules is not always present. 
The only constant and certain 
characteristic of anthrax is the 
presence of bacilli among the 
blood corpuscules. JHT ^fPgg|pi|^ ^9" 

To definitely ascertain if an I 
animal has died of anthrax it is 
necessary to obtain, within 12 
hours after death,* a small quan- 
tity of blood from the heart and 
subject it to a microscopic ex- 
amination at the hands of an ex- 
perienced bacteriologist. The 
presence of anthrax bacilli de- 
notes the disease. 

As a contributory measure 

J jb'IG. 52. 

against the spread of anthrax, 

carcasses should be burnt, or a small plot of ground set apart as a cemetery, 
enclosed by walls whose foundations are deep enough to prevent the surface 
water from penetrating to the adjoining land, and high enough to exclude 




: than 12 hours have elapsed, other organisms of a misleading nature may have developed. 



cattle. But the only effectual preventive remedy against the contagion of 
anthrax is the inoculation of individual animals with Pasteur's Anthrax 
Vaccine, of winch we ivill now proceed to speak. 



III.— PASTEUR'S ANTHRAX VACCINE. 



The heavy mortality following an outbreak of anthrax, and the rapidity 
with which the disease runs its course when once its victim is selected, 
caused V Pasteur to direct his efforts to the discovery of a preventive 
remedy rather than a cure. It had long ago been proved that human beings 
could be vaccinated against small-pox, and it seemed to M. Pasteur that it 
might also be possible to vaccinate animals against anthrax. 

In 1880 Pasteur discovered the first example of a malady (chicken 
cholera) produced by a special microbe, which by a peculiar process could 
be deprived of a part of its virulence, and then fowls might be inoculated 
with it without danger. By means of this attenuated virus the disease in a 
mild form was communicated to the fowl; and, on account of this mild and 
harmless attack, the fowl was protected against the disease, even if presented 
in an acute form. 

M. Pasteur thought that the process that had enabled him to attenuate 
the action of the chicken cholera microbe was likely to be a general process 
for the attenuation of the virulence of microbes in general. The anthrax 
bat alius was known; by cultivating it an antidote might be obtained. By 
following this reasoning Pasteur discovered a "vaccine" for anthrax. 

On February 28, 1881, Pasteur, in his own name and in those of his 
two fellow-workers, Messrs Chamberland and Roux, communicated his 
great discovery to the Academy of Sciences, Paris. The theory of vaccination 
against anthrax was established. 

The first public experiment was made at Pouilly-le-Fort, France, in the 
early part of 1881, and was remarkably successful. Sixty animals were 
obtained; 31 were vaccinated, 29 not. The whole number were then 
inoculated with crude virus. The 31 vaccinated animals were not in the 
least inconvenienced, while of the 29 not vaccinated 25 died and the 
remainder suffered from intense fever, exhibited large swellings and were 
generally very sick. More than 200 persons witnessed the result of the 
experiments — Government officials, senators, counsellors, journalists, phy- 
sicians, veterinary surgeons and stock-owners. Great enthusiasm was 
manifested at the truly remarkable results obtained, and which fully con- 
firmed M. Pasteur's assertions. Pasteur s method of vaccination against 
anthrax was practicable. During the years 1881 and 1882 a series of 
experiments on a large scale was carried out in France and in several 
other European countries. These experiments confirmed the results 
previously arrived at, and irrefutably proved the efficacy of Pasteur's 



Anthrax Vaccine. The average annual mortality from anthrax was i$%; 
it was proved by these experiments that the mortality could by vaccination 
be practically reduced to zero. The year 1882 saw the close of the ex- 
perimental stage and the Pasteur method entered the field of public utility. 



IV.— PRACTICAL RESULTS. 
PASTEUR'S SYSTEM OF VACCINATION AQAINST ANTHRAX. 

Vaccination consists in two inoculations, the first being destined to 
prepare the animal to receive the second, twelve days being allowed to 
elapse between the two inoculations. The injection is made in the neck 
or shoulder of cattle, horses and mules, and on the inside of the thigh of 
sheep and goats. The dose for the former consists in }£ c. c. (about 4 
minims) of the vaccine lymph, and for the latter yi c. c. 

Vaccination, being a preventive remedy, it should, to insure its full 
effects, be practised upon healthy animals, that is to say, upon herds that 
are not already under the influence of the disease. If practised upon a 
herd into which anthrax has already made inroads, the animals may con- 
tinue to die until the vaccination be complete, that is until twelve days 
after the second inoculation. However, herds already decimated by anthrax 
have been vaccinated, the disease has at once diminished, and disappeared 
entirely after the second inoculation. But vaccination, if delayed, will not 
prevent the death of animals already impregnated with the anthrax bacilli. 

Several millions of inoculations have now been made with Pasteur's 
Anthrax Vaccine, and the results obtained from its use cannot for a 
moment be questioned. The mortality from anthrax among vaccinated 
animals is practically nil. Vaccination is a simple, harmless and inexpensive 
operation. 

I. France. — Inasmuch as it was in France that Pasteur's Anthrax 
Vaccine was discovered, it is only natural that it should have been in that 
country that vaccination was first practised. The legislature sanctioned 
vaccination and in certain cases imposed it. The ministerial decree of 
June 22, 1882, containing regulations for the execution of the laws regard- 
ing sanitary inspection of animals stipulates (1) that the quarantine of 
pastures, etc., declared infected should not apply to animals that had just been 
vaccinated, and (2) that animals whose importation was prohibited might enter 
the country if vaccinated immediately. Anthrax was declared a contagious 
disease by decree of July 23, 1888, and regulations were issued for the 
inspection of herds affected, the destruction of animals attacked and the 
special disposition of animals exposed to contagion; but animals that had 
been vaccinated were especially exempted. 

Up to this date reports have been received from veterinary surgeons 
giving the result of the vaccination of over 4,000,000 animals in France. 



8 

These returns show that the annual mortality from anthrax has dropped from 
r>.6</< in sheep, and from j% to 0.18% in cattle. Two millions of dollars 
have in this way been saved to French stock-owners alone! 

In 1886 Pasteur's Anthrax Vaccine began its commercial life. In that 
year was formed the Soci te du Vaccin Charbonneux Pasteur (Pasteur Anthrax 
Vaccine Company), of Paris, and of which M. Chamberland, Pasteur's col- 
laborator in the discovery of anthrax vaccine, is technical director. The 
company acquired from Messrs. Pasteur, Chamberland and Roux the sole 
right to establish laboratories for the preparation and sale of Pasteur's 
Anthrax Vaccine in all countries outside of France, the latter undertaking 
to supply the necessary cultures. Messrs. Pasteur, Chamberland and Roux 
are now the chiefs of the celebrated Paris Pasteur Institute. 

A si her medal was awarded to the Pasteur Company at the Paris Ex- 
position in 1889. Hygienic Section, Group VI, Class 64. 

It is important to note that the French Live Stock Insurance Company, 
"L'Avenir" requires a certificate of vaccination before covering the risk of 
death from anthrax. This condition practically renders vaccination com- 
pulsory. 

II. Austro-Hungary. — In Austro-Hungary anthrax (milzbrand) was 
very prevalent, the mortality from the disease among live stock being 
from 10 to 60 per cent. The initial experiments were conducted in 1881, 
at the Buda-Pesth Veterinary Institute, under the auspices of the Ministry 

Lgriculture. These experiments were completely successful and con- 
firmed the absolute efficacy of Pasteur's method of vaccination against 
anthrax. Pasteur's Anthrax Ya< < ine is now extensively used in these 
countries. A laboratory was established in Vienna in 1886 and transferred 
to Buda-Pesth in 1890. In 1887, 57,864 inoculations were made; in 1894 
(up to 20th August) 946,693 vaccinations were effected. Over four millions 
of animals have been vaccinated in Austro-Hungary, and more than 1,000 
Sto< k-owners have availed themselves of the benefits of vaccination. The 
mortality from anthrax has now dropped to 0.1SI for sheep, o. 06% for cattle, 
and to o.ogl for horses. 

The laboratory in Buda-Pesth is under the patronage of the Royal 
Hungarian Ministry of Agriculture; it prepares the lymph from cultures 
received from the Paris Pasteur Institute. Instruction in animal vaccina- 
tion is given to veterinary surgeons and to pupils at the Royal Veterinary 
Academy, Buda-Pesth. 

III. Germany. — The German cattle industry was a great sufferer from 
anthrax (milzbrand). In the spring of 1882, the Minister of Agriculture 
appointed a commission to conduct experiments with Pasteur's Anthrax 

ine. The experiments took place at Packisch and Borischutz in the 
months of April and May, 1882, and the reports of the commission once 
more confirmed the advantages and value of Pasteur's method of vaccina- 
tion against anthrax. The commis^on was of the opinion that vaccination 



would be economical and advantageous. Vaccination against anthrax 
became so general in Germany that at the beginning of 1894 a laboratory 
for the preparation of the vaccine was established in Stuttgart. The 
laboratory was placed by the Government under the control and super- 
vision of the Wurtemberg Royal Medical College. 

IV. Italy. — In Italy anthrax was extensively known. It raged prin- 
cipally among cattle, and the annual loss was from 10 to 20%. In 1882 the 
Minister of Agriculture appointed a commission to study the question. One 
of its members, M. Perroncito, visited the Pasteur Laboratory in Paris, and 
upon his return to Italy made extensive experiments which confirmed the 
value and efficacy of vaccination by Pasteur's method. The Italian 
government recommended its use. A laboratory for the preparation of the 
vaccine was established at Turin in 1S87. In 1894 it was purchased by 
the Italian government and transferred to Rome. The cultures are supplied 
by the Paris Pasteur Institute to the Public Health Department of the 
Italian Ministry of the Interior which prepares and distributes the vaccine. 
A ministerial circular, dated Rome, October 25, 1894, contains directions 
and fixes the veterinary surgeons' fees for vaccinating. 

V. Belgium. — In 1882 experiments with Pasteur's Anthrax Vaccine 
took place at Herve before the governmental and provincial authorities, 
the President of the Royal Agricultural Society of Eastern Belgium, the 
Clinical Professor of the Veterinary College, the delegates of several medi- 
cal and surgical societies, and numerous veterinary surgeons and stock- 
owners. The experiments once more confirmed the results already obtained 
in France and elsewhere, namely, that Pasteur's method of. vaccination 
teas a certain preventive against anthrax in herbivorous animals. Since 
that date Pasteur's Vaccine has been regularly obtained from the Pasteur 
Company in Paris, and used with uniform success. 

VI. Spain. — In this country anthrax prevailed among cattle, sheep and 
horses, the mortality being about 20^. In the year 1882, 2,400 doses were 
supplied to Spain; in 1886 the number of doses was over 40,000 and has 
since gradually increased. As a result of the use of Pasteur s Anthrax 
Vaccine the mortality from anthrax in Spain is now below 1%. 

VII. Russia. — Siberian pest (anthrax) has long been a cause of terrible 
loss among domestic animals throughout the Russian empire. In July, 
1894, experiments were made with Pasteur's Anthrax Vaccine which 
resulted in the confirmation of M. Pasteur's representations, namely: that 
animals vaccinated in accordance with his method were protected against 
anthrax. The use of Pasteur's Anthrax Vaccine was in due course 
authorized by the Russian government, and inoculations are practised on 
a considerable scale. A laboratory is being established at Nijni Novgorod 
for the preparation and sale of Pasteur's Anthrax Vaccine, the cultures 
being supplied by the Paris Pasteur Institute. (Imperial Decree No. 
4,427, August 26, 1894.) There is already a very large demand for vaccine 



from all parts of the country, and Pasteur's method of inoculation appears 
destined to become the popular preventive remedy against the deadly 
scourge, Siberian pest, which has heretofore occasioned such disastrous 
losses throughout the Russian empire. 

VIII. Great Britain. — In some parts of Great Britain anthrax has a 
constant existence, but it is only during recent years that outbreaks have 
occured to any appreciable extent. In the year 1889 the reported losses 
from anthrax were 288 animals, while in 1893 the number had gradually 
increased to n 75. (Report of the Director of the Veterinary Department 
of the English Board of Agriculture, 1893.) 

Pasteur's Anthrax Vaccine has hitherto been used in Great Britain to 
only a limited extent, but, owing to the continued increase of anthrax, the 
attention of the government authorities, veterinarians and stock-owners has 
recently been directed to the subject. Great interest is now manifested in 

teur's Anthrax Vaccine throughout the country, and the President of 
the Board of Agriculture has taken steps to obtain reports respecting the 
feasibility of inoculation for anthrax and as to the measures adopted in 
other countries to prevent the spread of the disease. 

All the leading English veterinarians recommend Pasteur's system of 
preventive inoculation, and there is no doubt but that Pasteur's Anthrax 
Vac< ine will soon be- in general use throughout Great Britain, it being 
re< ognized that the only efficacious means of checking anthrax must be of 
.1 prote< tive character. 

IX. Australasia. — Cumberland disease causes terrible ravages in 
Australia, the annual mortality in many districts being between jo and 40% 
representing a loss of about 200,000 animals per annum. In 1888 the 
dreaded Cumberland disease was proved to be nothing more nor less than 
anthrax. At the request of the New South Wales government a committee 
was formed for the purpose of ascertaining the practical value of Pasteur's 
method of vaccination against anthrax. The demonstration took place at 
Junee, in October, 18S8. The efficacy of Pasteur's Anthrax Vaccine was 
proved and the committee unanimously recommended its adoption and use. 

In July, 1890, a laboratory was established at Rodd Island, near 
Sydney. N. S. W., where the lymph is prepared from the cultures sent from 
the Paris Pasteur Institute. In the first eighteen months 250,000 animals 
were vaccinated in Australia, while in the year 1893, 119,000 were success- 
fully vaccinated in New South Wales alone. The constantly increasing 
demand for lymph gives a correct idea of the value of Pasteur's Anthrax 
Vaccine. 

In March, 1S93, the United States Consul-General in Australia was 
directed by the State Department at Washington to institute inquiries 
regarding the use of Pasteur's Anthrax Vaccine in Australia. The report, 
dated July 24, 1893, was a carefully prepared document of 24 pages and 
showed that Australian stock-owners recognized the value of Pasteur's 



Anthrax Vaccine, were strongly in favor of it, and that its use was becoming 
general. 

X. Other Countries. — In addition to the countries already mentioned, 
Pasteur's Anthrax Vaccine is being used with the same successful results 
in South America and the "West Indies. After preliminary inquiries by the 
British Ambassador at Paris M. Saint-Marie, of the Pasteur Institute, was, 
at the request of the Viceroy of India, sent to Calcutta to establish a 
laboratory and otherwise assist in the introduction of Pasteur's Anthrax 
Vaccine in India, where anthrax is known under the name of "Loodiana 
disease." 

In August, 1S94, the Sultan of Turkey sanctioned the establishment 
within his dominions of a laboratory for the preparation of Pasteur's 
Anthrax Vaccine, thereby rendering inestimable service to the Turkish 
farmers by enabling them to combat the terrible disease by means of 
Pasteur's Anthrax Vaccine. 



From the foregoing resume it will be seen to what a large extent 
Pasteur's method of vaccination against anthrax is resorted to, and what 
a notable reduction in mortality has followed its use. Step by step the 
vaccine has been introduced into all European countries and Australia. 
Experiments and practical demonstrations have been made with the same 
unvarying result — it was proved that the vaccinated animal was protected 
against anthrax. Several laboratories are preparing the vaccine, namely: 
in Paris, Stuttgart, Buda-Pesth, Rome, Nijni- Novgorod and Sydney 
(Australia). Several millions of animals, cattle, horses, mules, sheep, goats 
and swine are being inoculated every year, and hundreds of thousands of 
dollars are annually saved to the owners and breeders of live stock. The 
efficacy and economy of vaccination as a preventive and protective remedy- 
is unquestionable Like all new discoveries it encountered its full share 
of opposition: scientists questioned its efficacy, veterinarians doubted its 
practicability, stock-owners feared its results. But Pasteur's Anthrax 
Vaccine has overcome all opposition and prejudice, and its scientific and 
commercial value is well established. Stock-owners, in their own interest, 
gladly avail themselves of its beneficial effects. 

Pasteur's Anthrax Vaccine is inexpensive in price, simple in its applica- 
tion and sure in its results. It is within the reach of all stock-owners, large 
and small. Its use is rapidly becoming universal, and we shall soon no 
longer hear of the heavy mortality from a disease so fatal as anthrax, now 
that it can be prevented by such a simple, safe and economic means as 
vaccination. 

Vaccination against anthrax in domestic animals is practically impera- 
tive, not only in districts where anthrax has a constant existence, but on 
every live-stock farm. Everything coming on the farm is liable to introduce 



>- 



the germ of the disease; and the only indication of the presence of the 
malady is sudden and unaccountable deaths, accompanied by heavy mor- 
tality. 

Moreover, pasture at present scrupulously avoided can again be utilized, 
as a vaccinated herd can safely graze on contaminated soil. 



'"-' 



The Pasteur Anthrax Vaccine (U. S. & Canada) Com- 
pany, L'd, are the sole agents in the United States and 
Canada for Pasteur's Anthrax Vaccine. 

Pending the establishment of a laboratory in the States, 
the vaccine is prepared at the Paris laboratory, which is 
under the supervision of M. Chamberland, who shares with 
Pasteur the honor of discovering the Anthrax Vaccine. 

Full particulars respecting the vaccine can be obtained 
upon application to the 

PASTEUR ANTHRAX VACCINE <u. s.& Canada, CO., L'D. 

369 BROADWAY, 

NEW YORK CITY. 



Test tubes and directions for obtaining blood for transmission for 
bacteriological examination will be sent upon application to the Pasteur 
Company. 



ANIMAL VACCINATION. 



VACCINATION 



AS A 



r reventiye rvemedy 



AGAINST 



DISEASES OF LIVE STOCK 



COPYRIGHTED 1895 



HAROLD SORBY 



Black Leg. 

Black Quarter. Symptomatic Anthrax, Charbon Symptoma- 
tique, Rauschbrand, etc. are a form of Anthrax and can be 
prevented by Vaccination. 

The Pasteur Company has just completed arrangements 
to supply Black Leg Vaccine. Full particulars upon 
application. 



The Pasteur Company desires to make arrangements 
with Veterinary Surgeons to undertake vaccinations in 
all parts of the States. 



PASTEUR ANTHRAX VACCINE u. s. & Canada, CO., L'D, 

369 BROADWAY, 

NEW YORK CITY. 



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